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advances in small molecule fluorescent probes for simultaneous imaging of two bioactive molecules in live

Yongqing Zhou, Xin Wang, Wei Zhang, Bo Tang, Ping Li

Frontiers of Chemical Science and Engineering 2022, Volume 16, Issue 1,   Pages 4-33 doi: 10.1007/s11705-021-2041-2

Abstract: its excellent performances, including high sensitivity and selectivity, noninvasive properties, real-time

Keywords： bioactive molecules     fluorescent probes     in living cells and in vivo     review    

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First Pig-to-Live Human Xenotransplant Produces Mixed Results

Sarah C.P. Williams

Engineering 2022, Volume 16, Issue 9,   Pages 6-8 doi: 10.1016/j.eng.2022.07.006

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Multi-level cross-layer protocol for end-to-end delay provisioning in wireless multimedia sensor networks Regular Papers-Research Articles

Hossein HADADIAN NEJAD YOUSEFI, Yousef SEIFI KAVIAN, Alimorad MAHMOUDI

Frontiers of Information Technology & Electronic Engineering 2019, Volume 20, Issue 9,   Pages 1266-1276 doi: 10.1631/FITEE.1700855

Abstract: When a node wants to send a packet, the MLCL protocol compares this minimum delay with the time to live

Keywords： Wireless multimedia sensor networks     Cross layering     Time to live     End-to-end delay     Quality of service    

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RR1 and RR2 gene deletion affects the immunogenicity of a live attenuated pseudorabies

Shijun YAN,He YAN,Chaolin ZHANG,Tongyan WANG,Qingyuan YANG,Zhe SUN,Yan XIAO,Feifei TAN,Xiangdong LI,Kegong TIAN

Frontiers of Agricultural Science and Engineering 2016, Volume 3, Issue 1,   Pages 81-86 doi: 10.15302/J-FASE-2016088

Abstract: As virulence-determining genes, and encode the small subunit and large subunit of viral ribonucleotide reductase (RR) in pseudorabies virus which have been extensively studied in mice. However, their role in pigs has not been adequately investigated. In this study, we deleted and genes based on a / / triple gene-deleted pseudorabies virus and tested its efficacy in pigs as a vaccine candidate. The rescued virus showed similar growth properties and plaque size as its parent strain. In an animal study, the virus could elicit humoral immune responses shown by generation of gB-specific antibodies and virus neutralizing antibodies. However, vaccination could not provide protection against virulent pseudorabies virus challenge since vaccinated pigs showed clinical pseudorabies-specific syndromes. The deficiency in protection may due to the generation of late and low levels of gB antibodies and virus neutralizing antibodies.

Keywords： pseudorabies virus     RR1 and RR2     ribonucleotide reductase     vaccine candidate    

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Attenuation mechanism of

Yuanqiang ZHENG,Yanchun SHI,Chang AN,Ruisheng LI,Dongjun LIU,Yuehua KE,Kairong MAO,Mingjuan YANG,Zeliang CHEN,Shorgan BOU

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 4,   Pages 331-340 doi: 10.15302/J-FASE-2014032

Abstract: Brucellosis is a worldwide zoonosis. Vaccination is the most efficient means to prevent and control brucellosis. The current licensed attenuated vaccines for animal use were developed by sequential passage in non-natural hosts that decreased virulence in its original hosts. The attenuation mechanism of these strains remains largely unknown. In the present study, we sequenced the genome of vaccine strain M5-10. Sequence analysis showed that a large number of genetic changes occurred in the vaccine strains. A total of 2854 genetic polymorphic sites, including 2548 SNP, 241 INDEL and 65 MNV were identified. Of the 2074 SNPs in coding regions, 1310 (63.2%) were non-synonymous SNPs. Gene number, percent and N/S ratios were disproportionally distributed among the cog categories. Genetic polymorphic sites were identified in genes of the operon, flagella synthesis, and virulence regulating systems. These data indicate that changes in some cog categories and virulence genes might result in the attenuation. These attenuation mechanisms also have implications for screening and development of new vaccine strains. The genetic changes in the genome represent candidate sites for differential diagnosis between these vaccine strains and other virulence ones. Transcription analysis of virulence genes showed that expression of , were reduced in M5-10 strain when compared with that in 16M. A duplex PCR targeting and was successfully used to differentiate between M5-10 and the virulent 16M strain. The genome re-sequencing technique represents a strong strategy not only for evaluation of vaccines, but also for development of new vaccines.

Keywords： Brucella     live attenuated vaccine     attenuation     differential diagnosis    

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Synthesis and properties of water-soluble 1,9-dialkyl-substituted BF

Dan Wu, Gonzalo Durán-Sampedro, Donal F. O’Shea

Frontiers of Chemical Science and Engineering 2020, Volume 14, Issue 1,   Pages 97-104 doi: 10.1007/s11705-019-1828-x

Abstract: Live cell microscopy imaging revealed efficient uptake and intracellular labelling of cells for bothNIR-active azadipyrromethene derivatives potentially useful as NIR fluorescence imaging probes for live

Keywords： NIR-fluorophores     live cell imaging     NIR-AZA    

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General expression for linear and nonlinear time series models

Ren HUANG, Feiyun XU, Ruwen CHEN

Frontiers of Mechanical Engineering 2009, Volume 4, Issue 1,   Pages 15-24 doi: 10.1007/s11465-009-0015-z

Abstract: The typical time series models such as ARMA, AR, and MA are founded on the normality and stationarityThis paper proposes a general expression for linear and nonlinear auto-regressive time series modelsThe modeling and prediction accuracy of the GNAR model is superior to the classical time series models

Keywords： linear and nonlinear     autoregressive model     system identification     time series analysis    

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Run-time system based on LinSERCANS and Soft-PLC

Cunfeng KANG, Chong WANG, Chunmin MA, Xudong HUANG, Renyuan FEI

Frontiers of Mechanical Engineering 2009, Volume 4, Issue 2,   Pages 120-124 doi: 10.1007/s11465-009-0019-8

Abstract: It is able to combine LinSERCANS with the Soft-PLC, and a run-time system is developed based on the interfacetechnology of the serial real-time communication system (SERCOS) and technology of soft-PLC.This run-time system has been used in all electronic injection molding machines and works very well.

Keywords： Soft-PLC     run-time system     LinSERCANS     dynamic link libraries    

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Two-stage scheduling on batch and single machines with limited waiting time constraint

Zhongshun SHI, Zewen HUANG, Leyuan SHI

Frontiers of Engineering Management 2017, Volume 4, Issue 3,   Pages 368-374 doi: 10.15302/J-FEM-2017034

Abstract: study addresses the problem of two-stage scheduling on batch and single machines with limited waiting time

Keywords： batch machine     flow shop     makespan     limited waiting time    

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Analyzing construction safety through time series methods

Houchen CAO, Yang Miang GOH

Frontiers of Engineering Management 2019, Volume 6, Issue 2,   Pages 262-274 doi: 10.1007/s42524-019-0015-6

Abstract: Various time series (TS) methods were adopted for identifying the leading indicators or predictors of

Keywords： time series     temporal     construction safety     leading indicators     accident prevention     forecasting    

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Propensity score-matched study and meta-analysis of cumulative outcomes of day 2/3 versus day 5/6 embryo transfers

Ye Yin, Ge Chen, Kezhen Li, Qiuyue Liao, Sijia Zhang, Nieying Ma, Jing Chen, Yan Zhang, Jihui Ai

Frontiers of Medicine 2017, Volume 11, Issue 4,   Pages 563-569 doi: 10.1007/s11684-017-0535-6

Abstract: The cumulative pregnancy rate (57.14% vs. 53.46%, OR 1.16, 95% CI 0.79–1.70) and cumulative liveThe mean cycles per live birth and mean days per live birth in the day 5/6 group were significantly lowerThis study demonstrated that day 5/6 embryo transfer is a more cost-effective and time-efficient policythan day 2/3 embryo transfer to produce a live baby.

Keywords： blastocyst     embryo transfer     cumulative pregnancy rate     cumulative live birth rate     IVF    

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Convergence to real-time decision making

James M. TIEN

Frontiers of Engineering Management 2020, Volume 7, Issue 2,   Pages 204-222 doi: 10.1007/s42524-019-0040-5

Abstract: Real-time decision making reflects the convergence of several digital technologies, including those concernedwith the promulgation of artificial intelligence and other advanced technologies that underpin real-timeMore specifically, real-time decision making can be depicted in terms of three converging dimensions:Internet of Things, decision making, and real-time.Real-time includes mobile networks, autonomous vehicles, and artificial general intelligence.

Keywords： real-time decision making     services     goods     ServGoods     Big Data     Internet of Things     artificial intelligence    

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Damage identification in connections of moment frames using time domain responses and an optimization

Frontiers of Structural and Civil Engineering 2021, Volume 15, Issue 4,   Pages 851-866 doi: 10.1007/s11709-021-0739-3

Abstract: this study, an optimization-based method for joint damage identification of moment frames using the time-domain

Keywords： damage identification     beam-to-column connection     time-domain response     optimization    

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origins of schedule management: the concepts used in planning, allocating, visualizing and managing time

Lynda M. BOURNE, Patrick WEAVER

Frontiers of Engineering Management 2018, Volume 5, Issue 2,   Pages 150-166 doi: 10.15302/J-FEM-2018012

Abstract: The first section of the paper looks at the development of concepts that allow the visualization of timethe emergence of computers, dynamic calculations and integrated data from the 1950s to the present time

Keywords： time management     scheduling     CPM     PERT     Gantt     Critical Path     bar chart    

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Sustainability of steel structures: towards an integrated approach to life-time engineering design

Raffaele LANDOLFO, Lucrezia CASCINI, Francesco PORTIOLI

Frontiers of Structural and Civil Engineering 2011, Volume 5, Issue 3,   Pages 304-314 doi: 10.1007/s01709-011-0123-9

Abstract: Indeed the current trend in civil engineering research is moving towards life-time engineering, withimplement integrated methodologies to consider as a whole all the sustainability requirements according to time-dependentof the concept of sustainability applied to constructions, this paper presents an overview of life-timemetal structures with respect to the degradation phenomena able to impair the structural capacity over timeFinally a proposal towards an integrated approach to life-time engineering design of steel structures

Keywords： sustainability     life-time engineering     performance based design     durability     metal structures    

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Title Author Date Type Operation

advances in small molecule fluorescent probes for simultaneous imaging of two bioactive molecules in live

Yongqing Zhou, Xin Wang, Wei Zhang, Bo Tang, Ping Li

Journal Article

First Pig-to-Live Human Xenotransplant Produces Mixed Results

Sarah C.P. Williams

Journal Article

Multi-level cross-layer protocol for end-to-end delay provisioning in wireless multimedia sensor networks

Hossein HADADIAN NEJAD YOUSEFI, Yousef SEIFI KAVIAN, Alimorad MAHMOUDI

Journal Article

RR1 and RR2 gene deletion affects the immunogenicity of a live attenuated pseudorabies

Shijun YAN,He YAN,Chaolin ZHANG,Tongyan WANG,Qingyuan YANG,Zhe SUN,Yan XIAO,Feifei TAN,Xiangdong LI,Kegong TIAN

Journal Article

Attenuation mechanism of

Yuanqiang ZHENG,Yanchun SHI,Chang AN,Ruisheng LI,Dongjun LIU,Yuehua KE,Kairong MAO,Mingjuan YANG,Zeliang CHEN,Shorgan BOU

Journal Article

Synthesis and properties of water-soluble 1,9-dialkyl-substituted BF

Dan Wu, Gonzalo Durán-Sampedro, Donal F. O’Shea

Journal Article

General expression for linear and nonlinear time series models

Ren HUANG, Feiyun XU, Ruwen CHEN

Journal Article

Run-time system based on LinSERCANS and Soft-PLC

Cunfeng KANG, Chong WANG, Chunmin MA, Xudong HUANG, Renyuan FEI

Journal Article

Two-stage scheduling on batch and single machines with limited waiting time constraint

Zhongshun SHI, Zewen HUANG, Leyuan SHI

Journal Article

Analyzing construction safety through time series methods

Houchen CAO, Yang Miang GOH

Journal Article

Propensity score-matched study and meta-analysis of cumulative outcomes of day 2/3 versus day 5/6 embryo transfers

Ye Yin, Ge Chen, Kezhen Li, Qiuyue Liao, Sijia Zhang, Nieying Ma, Jing Chen, Yan Zhang, Jihui Ai

Journal Article

Convergence to real-time decision making

James M. TIEN

Journal Article

Damage identification in connections of moment frames using time domain responses and an optimization

Journal Article

origins of schedule management: the concepts used in planning, allocating, visualizing and managing time

Lynda M. BOURNE, Patrick WEAVER

Journal Article

Sustainability of steel structures: towards an integrated approach to life-time engineering design

Raffaele LANDOLFO, Lucrezia CASCINI, Francesco PORTIOLI

Journal Article